Journal: PLoS ONE

Article Title: Osmostress Induces Autophosphorylation of Hog1 via a C-Terminal Regulatory Region That Is Conserved in p38α

doi: 10.1371/journal.pone.0044749

Figure Lengend Snippet: A. Phosphorylation levels of p38α wild type (p38α) and p38α missing amino acids Y342–F348 (p38α (Y342–F348)Δ ) were tested when expressed in hog1 Δ pbs2 Δ strain, before (−) and 10 minutes after (+) exposure to 1 M NaCl. B. Human embryonic kidney 293 cells were trasnfected with the indicated plasmids. Forty-eight hours after transfections cells lysates were prepared and analyzed by Western blots using the indicated antibodies. All p38 constructs are HA-tagged whereas MKK6 EE is flag-tagged. MK2 ( = MAPKAPK2) is a substrate of p38α.

Article Snippet: Antibodies used were: anti-phospho-p38 (cell signaling #9211), anti HA antibodies (12CA5 monoclonal), anti-MAPKAPK2 (cell signaling #3042), anti-phospho MAPKAPK2 (cell signaling, #3007) and anti-flag (sigma, A2220).

Techniques: Phospho-proteomics, Transfection, Western Blot, Construct

Journal: EMBO Reports

Article Title: Promyelocytic leukemia protein targets MK2 to promote cytotoxicity

doi: 10.15252/embr.202052254

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal anti‐MAPKAPK2 , Invitrogen , #PA5‐17729, RRID:AB_10979499 , WB 1:2,000 (Blocking buffer: SuperBlock™ T20) IP 1:200 IF 1:200.

Techniques: Generated, Transgenic Assay, Recombinant, Plasmid Preparation, Blocking Assay, Transfection, Antibody Labeling, Software, Cell Viability Assay